› 5yje

X-ray diffraction
2.45Å resolution

Crystal structure of HIRA(644-1017)

Released:
DOI: 10.2210/pdb5yje/pdb
PDB entry 5yje coloured by chain, front view.
PDB entry 5yje coloured by chain, side view.
PDB entry 5yje coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit.
Ray-Gallet D, Ricketts MD, Sato Y, Gupta K, Boyarchuk E, Senda T, Marmorstein R, Almouzni G
Nat Commun 9 3103 (2018)
PMID: 30082790

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein HIRA Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 380 amino acids
Theoretical weight: 41.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P54198 (Residues: 644-1017; Coverage: 37%)
Gene names: DGCR1, HIR, HIRA, TUPLE1
Sequence domains: TUP1-like enhancer of split

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 149.99Å b: 86.8Å c: 99.15Å
α: 90° β: 102.49° γ: 90°
R-values:
R R work R free
0.204 0.202 0.256
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The roles of histone variants in fine-tuning chromatin organization and function.
Martire et al. (2020)
4 more

2 mentions without citation

Molecular basis for chromatin assembly and modification by multiprotein complexes.
Ricketts et al. (2019)
1 more