› 5yjf

X-ray diffraction
2.49Å resolution

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + nicotinamide = S-adenosyl-L-homocysteine + 1-methylnicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154063 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 284 amino acids
Theoretical weight: 31.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40261 (Residues: 1-264; Coverage: 100%)
Gene name: NNMT
Sequence domains: NNMT/PNMT/TEMT family
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
1 bound ligand:
Ligand 8WO 4 x 8WO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 60.323Å b: 132.976Å c: 61.317Å
α: 90° β: 103.57° γ: 90°
R-values:
R R work R free
0.227 0.224 0.286
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Niacin in the Central Nervous System: An Update of Biological Aspects and Clinical Applications.
Gasperi et al. (2019)
17 more

2 mentions without citation

Mechanisms and inhibitors of nicotinamide N-methyltransferase.
Iyamu et al. (2021)
1 more