› 5yt3

X-ray diffraction
2.9Å resolution

Structure of the Human Mitogen-Activated Protein Kinase Kinase 1 S218D and S222D mutant

Released:
DOI: 10.2210/pdb5yt3/pdb
PDB entry 5yt3 coloured by chain, front view.
PDB entry 5yt3 coloured by chain, side view.
PDB entry 5yt3 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Nakae S, Doko K, Tada T, Shirai T

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 350 amino acids
Theoretical weight: 39.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02750 (Residues: 39-382; Coverage: 88%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
Structure domains: Transferase(Phosphotransferase) domain 1

Ligands and Environments

2 bound ligands:
Ligand ANP 4 x ANP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 132.17Å b: 132.55Å c: 91.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.209 0.289
Expression system: Escherichia coli

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