› 5yuf

X-ray diffraction
1.6Å resolution

Crystal Structure of PML RING tetramer

Released:
DOI: 10.2210/pdb5yuf/pdb
PDB entry 5yuf coloured by chain, front view.
PDB entry 5yuf coloured by chain, side view.
PDB entry 5yuf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
RING tetramerization is required for nuclear body biogenesis and PML sumoylation.
Wang P, Benhenda S, Wu H, Lallemand-Breitenbach V, Zhen T, Jollivet F, Peres L, Li Y, Chen SJ, Chen Z, de Thé H, Meng G
Nat Commun 9 1277 (2018)
PMID: 29599493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151547 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PML Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 51 amino acids
Theoretical weight: 5.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P29590 (Residues: 49-99; Coverage: 6%)
Gene names: MYL, PML, PP8675, RNF71, TRIM19
Sequence domains: Zinc finger, C3HC4 type (RING finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 38.51Å b: 84.69Å c: 86.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

Membraneless nuclear organelles and the search for phases within phases.
Sawyer et al. (2019)
19 more

4 mentions without citation

Does it take two to tango? RING domain self-association and activity in TRIM E3 ubiquitin ligases.
Fiorentini et al. (2020)
3 more