› 5yvg

X-ray diffraction
4.05Å resolution

Crystal structure of Karyopherin beta2 in complex with FUS(full length)

Released:
DOI: 10.2210/pdb5yvg/pdb
PDB entry 5yvg coloured by chain, front view.
PDB entry 5yvg coloured by chain, side view.
PDB entry 5yvg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites.
Yoshizawa T, Ali R, Jiou J, Fung HYJ, Burke KA, Kim SJ, Lin Y, Peeples WB, Saltzberg D, Soniat M, Baumhardt JM, Oldenbourg R, Sali A, Fawzi NL, Rosen MK, Chook YM
Cell 173 693-705.e22 (2018)
PMID: 29677513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152978 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transportin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 868 amino acids
Theoretical weight: 98.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92973 (Residues: 9-344, 376-898; Coverage: 96%)
Gene names: KPNB2, MIP1, TNPO1, TRN
Sequence domains:
RNA-binding protein FUS Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 528 amino acids
Theoretical weight: 53.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35637 (Residues: 1-526; Coverage: 100%)
Gene names: FUS, TLS
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 108.219Å b: 145.015Å c: 108.681Å
α: 90° β: 94.552° γ: 90°
R-values:
R R work R free
0.365 0.25 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

79 review citations

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.
Alberti et al. (2019)
78 more

5 mentions without citation

Transportin-1: A Nuclear Import Receptor with Moonlighting Functions.
Mboukou et al. (2021)
4 more