› 5yxh

X-ray diffraction
2.04Å resolution

Structure of Rheb-GDP

Released:
DOI: 10.2210/pdb5yxh/pdb
PDB entry 5yxh coloured by chain, front view.
PDB entry 5yxh coloured by chain, side view.
PDB entry 5yxh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A small molecule inhibitor of Rheb selectively targets mTORC1 signaling.
Mahoney SJ, Narayan S, Molz L, Berstler LA, Kang SA, Vlasuk GP, Saiah E
Nat Commun 9 548 (2018)
PMID: 29416044

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172270 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding protein Rheb Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 171 amino acids
Theoretical weight: 19.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15382 (Residues: 2-169; Coverage: 91%)
Gene names: RHEB, RHEB2
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GDP 4 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 50.166Å b: 58.963Å c: 64.345Å
α: 110.53° β: 90.03° γ: 108.16°
R-values:
R R work R free
0.246 0.244 0.299
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Molecular mechanisms of dietary restriction promoting health and longevity.
Green et al. (2022)
19 more

1 mention without citation

A small molecule inhibitor of Rheb selectively targets mTORC1 signaling.
Mahoney et al. (2018)