› 5zc6

Solution NMR

Solution structure of H-RasT35S mutant protein in complex with KBFM123

Released:
DOI: 10.2210/pdb5zc6/pdb
PDB entry 5zc6 coloured by chain, ensemble of 15 models, front view.
PDB entry 5zc6 coloured by chain, ensemble of 15 models, side view.
PDB entry 5zc6 coloured by chain, ensemble of 15 models, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Basis for Allosteric Inhibition of GTP-Bound H-Ras Protein by a Small-Molecule Compound Carrying a Naphthalene Ring.
Matsumoto S, Hiraga T, Hayashi Y, Yoshikawa Y, Tsuda C, Araki M, Neya M, Shima F, Kataoka T
Biochemistry 57 5350-5358 (2018)
PMID: 30141910

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GNP 1 x GNP
Ligand KBF 1 x KBF
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 0%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

1H, 15N backbone assignment and comparative analysis of the wild type and G12C, G12D, G12V mutants of K-Ras bound to GDP at physiological pH.
Pálfy et al. (2020)
1 more

1 mention without citation

Therapeutic Targeting the Allosteric Cysteinome of RAS and Kinase Families.
Li et al. (2022)