› 5zmc

X-ray diffraction
2.99Å resolution

Structural Basis for Reactivation of -146C>T Mutant TERT Promoter by cooperative binding of p52 and ETS1/2

Released:
DOI: 10.2210/pdb5zmc/pdb
PDB entry 5zmc coloured by chain, front view.
PDB entry 5zmc coloured by chain, side view.
PDB entry 5zmc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for reactivating the mutant TERT promoter by cooperative binding of p52 and ETS1.
Xu X, Li Y, Bharath SR, Ozturk MB, Bowler MW, Loo BZL, Tergaonkar V, Song H
Nat Commun 9 3183 (2018)
PMID: 30093619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119521 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Protein C-ets-1 Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 13.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14921 (Residues: 331-441; Coverage: 25%)
Gene names: ETS1, EWSR2
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Nuclear factor NF-kappa-B p52 subunit Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 33.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00653 (Residues: 35-329; Coverage: 33%)
Gene names: LYT10, NFKB2
Sequence domains:
Structure domains: Immunoglobulins
DNA (5'-D(P*CP*GP*GP*GP*GP*AP*CP*CP*CP*GP*GP*AP*AP*GP*GP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 5.01 KDa
DNA (5'-D(P*GP*CP*CP*CP*TP*TP*CP*CP*GP*GP*GP*TP*CP*CP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.79 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-1
Spacegroup: P4122
Unit cell:
a: 71.414Å b: 71.414Å c: 262.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.259 0.288
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Small Molecule NF-κB Pathway Inhibitors in Clinic.
Ramadass et al. (2020)
11 more

2 mentions without citation

Structural basis for reactivating the mutant TERT promoter by cooperative binding of p52 and ETS1.
Xu et al. (2018)
1 more