5znr

X-ray diffraction
3.2Å resolution

Crystal structure of PtSHL in complex with an H3K27me3 peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-110822 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PHD-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 24.89 KDa
Source organism: Populus trichocarpa
Expression system: Escherichia coli
UniProt:
  • Canonical: B9H0V2 (Residues: 1-215; Coverage: 100%)
Gene name: POPTR_004G159900
Sequence domains:
Histone H3.1 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 17 amino acids
Theoretical weight: 1.67 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: P59226 (Residues: 21-37; Coverage: 13%)
Gene names: At1g09200, At3g27360, At5g10390, At5g10400, At5g65360, F12B17_250, F12B17_260, HTR1, HTR13, HTR2, HTR3, HTR9, K1G2.8, MNA5.9, T12M4.9

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 43.717Å b: 110.315Å c: 58.643Å
α: 90° β: 96.64° γ: 90°
R-values:
R R work R free
0.209 0.206 0.232
Expression systems:
  • Escherichia coli
  • Not provided