› 5a0l

X-ray diffraction
1.35Å resolution

N-terminal thioester domain of fibronectin-binding protein SfbI from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb5a0l/pdb
PDB entry 5a0l coloured by chain, front view.
PDB entry 5a0l coloured by chain, side view.
PDB entry 5a0l coloured by chain, top view.
Source organism: Streptococcus pyogenes MGAS2096
Primary publication:
An internal thioester in a pathogen surface protein mediates covalent host binding.
Walden M, Edwards JM, Dziewulska AM, Bergmann R, Saalbach G, Kan SY, Miller OK, Weckener M, Jackson RJ, Shirran SL, Botting CH, Florence GJ, Rohde M, Banfield MJ, Schwarz-Linek U
Elife 4 (2015)
PMID: 26032562

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioester domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.18 KDa
Source organism: Streptococcus pyogenes MGAS2096
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0M3KL43 (Residues: 3-210; Coverage: 99%)
Sequence domains: Thioester domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I41
Unit cell:
a: 165.12Å b: 165.12Å c: 42.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.131 0.153
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Engineering of Metabolic Pathways by Artificial Enzyme Channels.
Pröschel et al. (2015)
5 more

2 mentions without citation

An internal thioester in a pathogen surface protein mediates covalent host binding.
Walden et al. (2015)
1 more