› 5a24

X-ray diffraction
1.5Å resolution

Crystal structure of Dionain-1, the major endopeptidase in the Venus flytrap digestive juice

Released:
DOI: 10.2210/pdb5a24/pdb
PDB entry 5a24 coloured by chain, front view.
PDB entry 5a24 coloured by chain, side view.
PDB entry 5a24 coloured by chain, top view.
Source organism: Dionaea muscipula
Primary publication:
Enzymatic and Structural Characterization of the Major Endopeptidase in the Venus Flytrap Digestion Fluid.
Risør MW, Thomsen LR, Sanggaard KW, Nielsen TA, Thøgersen IB, Lukassen MV, Rossen L, Garcia-Ferrer I, Guevara T, Scavenius C, Meinjohanns E, Gomis-Rüth FX, Enghild JJ
J Biol Chem 291 2271-87 (2016)
PMID: 26627834

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dionain-1 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 23.04 KDa
Source organism: Dionaea muscipula
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A0E3GLN3 (Residues: 131-352; Coverage: 69%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

2 bound ligands:
Ligand E64 1 x E64
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 73.85Å b: 47.79Å c: 57.79Å
α: 90° β: 92.44° γ: 90°
R-values:
R R work R free
0.158 0.158 0.172
Expression system: Komagataella pastoris

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Citations

7 citation in other articles

The role of electrical and jasmonate signalling in the recognition of captured prey in the carnivorous sundew plant Drosera capensis.
Krausko et al. (2017)
6 more

5 mentions without citation

Long-read sequencing uncovers the adaptive topography of a carnivorous plant genome.
Lan et al. (2017)
4 more