› 5a2g

X-ray diffraction
1.9Å resolution

An esterase from anaerobic Clostridium hathewayi can hydrolyze aliphatic aromatic polyesters

Released:
DOI: 10.2210/pdb5a2g/pdb
PDB entry 5a2g coloured by chain, front view.
PDB entry 5a2g coloured by chain, side view.
PDB entry 5a2g coloured by chain, top view.
Source organism: Hungatella hathewayi DSM 13479
Primary publication:
An Esterase from Anaerobic Clostridium hathewayi Can Hydrolyze Aliphatic-Aromatic Polyesters.
Perz V, Hromic A, Baumschlager A, Steinkellner G, Pavkov-Keller T, Gruber K, Bleymaier K, Zitzenbacher S, Zankel A, Mayrhofer C, Sinkel C, Kueper U, Schlegel K, Ribitsch D, Guebitz GM
Environ Sci Technol 50 2899-907 (2016)
PMID: 26878094

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112462 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 522 amino acids
Theoretical weight: 58.28 KDa
Source organism: Hungatella hathewayi DSM 13479
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D3AU79 (Residues: 1-414; Coverage: 100%)
Gene name: CLOSTHATH_07193
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, DESY BEAMLINE P11
Spacegroup: P21
Unit cell:
a: 62.05Å b: 241.83Å c: 83.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Surface engineering of polyester-degrading enzymes to improve efficiency and tune specificity.
Biundo et al. (2018)
1 more

1 mention without citation

Display of a novel carboxylesterase CarCby on Escherichia coli cell surface for carbaryl pesticide bioremediation.
Liu et al. (2022)