› 5a4v

X-ray diffraction
2.38Å resolution

AtGSTF2 from Arabidopsis thaliana in complex with quercetin

Released:
DOI: 10.2210/pdb5a4v/pdb
PDB entry 5a4v coloured by chain, front view.
PDB entry 5a4v coloured by chain, side view.
PDB entry 5a4v coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural evidence for Arabidopsis glutathione transferase AtGSTF2 functioning as a transporter of small organic ligands.
Ahmad L, Rylott EL, Bruce NC, Edwards R, Grogan G
FEBS Open Bio 7 122-132 (2017)
PMID: 28174680

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase F2 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 212 amino acids
Theoretical weight: 24.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46422 (Residues: 1-212; Coverage: 100%)
Gene names: At4g02520, GSTF2, PM24.1, T10P11.18
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 6 x ACT
Ligand QUE 3 x QUE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 88.03Å b: 94.83Å c: 153.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Glutathione S-transferase: a versatile protein family.
Vaish et al. (2020)
2 more

3 mentions without citation

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.
Sylvestre-Gonon et al. (2019)
2 more