› 5a5k

X-ray diffraction
2.77Å resolution

AtGSTF2 from Arabidopsis thaliana in complex with camalexin

Released:
DOI: 10.2210/pdb5a5k/pdb
PDB entry 5a5k coloured by chain, front view.
PDB entry 5a5k coloured by chain, side view.
PDB entry 5a5k coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural evidence for Arabidopsis glutathione transferase AtGSTF2 functioning as a transporter of small organic ligands.
Ahmad L, Rylott EL, Bruce NC, Edwards R, Grogan G
FEBS Open Bio 7 122-132 (2017)
PMID: 28174680

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase F2 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X
Length: 212 amino acids
Theoretical weight: 24.16 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46422 (Residues: 1-212; Coverage: 100%)
Gene names: At4g02520, GSTF2, PM24.1, T10P11.18
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 7WB 24 x 7WB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 97.1Å b: 113.72Å c: 132.02Å
α: 83.65° β: 79.47° γ: 65.85°
R-values:
R R work R free
0.252 0.25 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Glutathione S-transferase: a versatile protein family.
Vaish et al. (2020)
2 more

2 mentions without citation

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.
Sylvestre-Gonon et al. (2019)
1 more