› 5a5y

X-ray diffraction
1.92Å resolution

Crystal structure of AtTTM3 in complex with tripolyphosphate and magnesium ion (form A)

Released:
DOI: 10.2210/pdb5a5y/pdb
PDB entry 5a5y coloured by chain, front view.
PDB entry 5a5y coloured by chain, side view.
PDB entry 5a5y coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.
Martinez J, Truffault V, Hothorn M
J Biol Chem 290 23348-60 (2015)
PMID: 26221030

Function and Biology Details

Reaction catalysed: 
Triphosphate + H(2)O = diphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triphosphate tunnel metalloenzyme 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 212 amino acids
Theoretical weight: 24.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SIY3 (Residues: 1-210; Coverage: 100%)
Gene names: At2g11890, F23M2.5, TTM3
Sequence domains: CYTH domain
Structure domains: Hypothetical Protein Pfu-838710-001

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand 3PO 2 x 3PO
Ligand EDO 12 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I422
Unit cell:
a: 136.334Å b: 136.334Å c: 144.202Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.153 0.198
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
1 more

1 mention without citation

Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.
Martinez et al. (2015)