› 5a62

X-ray diffraction
1.5Å resolution

Hydrolytic potential of the ammonia-oxidizing Thaumarchaeon Nitrososphaera gargenis - crystal structure and activity profiles of carboxylesterases linked to their metabolic function

Released:
DOI: 10.2210/pdb5a62/pdb
PDB entry 5a62 coloured by chain, front view.
PDB entry 5a62 coloured by chain, side view.
PDB entry 5a62 coloured by chain, top view.
Source organism: Candidatus Nitrososphaera gargensis
Entry authors: Chow J, Kaljunen H, Nittinger E, Spieck E, Rarey M, Mueller-Dieckmann J, Streit WR

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125480 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.14 KDa
Source organism: Candidatus Nitrososphaera gargensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: K0IM51 (Residues: 1-277; Coverage: 100%)
Gene name: Ngar_c30910
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Unit cell:
a: 50.947Å b: 65.461Å c: 84.591Å
α: 90° β: 104.17° γ: 90°
R-values:
R R work R free
0.143 0.142 0.163
Expression system: Escherichia coli BL21(DE3)

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