› 5a68

X-ray diffraction
1.67Å resolution

Crystal structure of the AtTTM3 product complex with two orthophosphates and manganese ions (form B)

Released:
DOI: 10.2210/pdb5a68/pdb
PDB entry 5a68 coloured by chain, front view.
PDB entry 5a68 coloured by chain, side view.
PDB entry 5a68 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.
Martinez J, Truffault V, Hothorn M
J Biol Chem 290 23348-60 (2015)
PMID: 26221030

Function and Biology Details

Reaction catalysed: 
Triphosphate + H(2)O = diphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triphosphate tunnel metalloenzyme 3 Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 24.32 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SIY3 (Residues: 1-210; Coverage: 100%)
Gene names: At2g11890, F23M2.5, TTM3
Sequence domains: CYTH domain
Structure domains: Hypothetical Protein Pfu-838710-001

Ligands and Environments

2 bound ligands:
Ligand MN 3 x MN
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 43.331Å b: 33.811Å c: 71.812Å
α: 90° β: 94.4° γ: 90°
R-values:
R R work R free
0.181 0.179 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

mRNA capping: biological functions and applications.
Ramanathan et al. (2016)
1 more

1 mention without citation

Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.
Martinez et al. (2015)