› 5a72

X-ray diffraction
2.6Å resolution

Crystal structure of the homing endonuclease I-CvuI in complex with its target (Sro1.3) in the presence of 2 mM Ca

Released:
DOI: 10.2210/pdb5a72/pdb
PDB entry 5a72 coloured by chain, front view.
PDB entry 5a72 coloured by chain, side view.
PDB entry 5a72 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.
Molina R, Redondo P, López-Méndez B, Villate M, Merino N, Blanco FJ, Valton J, Grizot S, Duchateau P, Prieto J, Montoya G
J Biol Chem 290 28727-36 (2015)
PMID: 26363068

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118392 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA endonuclease I-CvuI Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 19.28 KDa
Source organism: Chlorella vulgaris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56347 (Residues: 2-161; Coverage: 99%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
24MER DNA, 5'-D(*DTP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*AP *DCP*GP*AP*CP*GP*TP*TP*CP*TP*GP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 51.179Å b: 94.21Å c: 51.34Å
α: 90° β: 104.83° γ: 90°
R-values:
R R work R free
0.168 0.161 0.233
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 mention without citation

Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.
Molina et al. (2015)