› 5ac6

X-ray diffraction
1.99Å resolution

S.enterica HisA mutant D10G, dup13-15, Q24L, G102A

Released:
DOI: 10.2210/pdb5ac6/pdb
PDB entry 5ac6 coloured by chain, front view.
PDB entry 5ac6 coloured by chain, side view.
PDB entry 5ac6 coloured by chain, top view.
Source organism: Salmonella enterica
Entry authors: Guo X, Soderholm A, Newton M, Nasvall J, Andersson D, Patrick W, Selmer M

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145288 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 27.43 KDa
Source organism: Salmonella enterica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10372 (Residues: 1-15, 17-245; Coverage: 100%)
Gene names: STM2076, hisA
Sequence domains: Histidine biosynthesis protein
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P6122
Unit cell:
a: 85.239Å b: 85.239Å c: 119.986Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.193 0.244
Expression system: Escherichia coli BL21(DE3)

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