› 5aem

X-ray diffraction
3.4Å resolution

Structure of t131 N-terminal TPR array

Released:
DOI: 10.2210/pdb5aem/pdb
PDB entry 5aem coloured by chain, front view.
PDB entry 5aem coloured by chain, side view.
PDB entry 5aem coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.
Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Müller CW
Nat Commun 6 7387 (2015)
PMID: 26060179

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor tau 131 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 447 amino acids
Theoretical weight: 52.37 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P33339 (Residues: 123-566; Coverage: 43%)
Gene names: PCF1, TFC4, YGR047C
Sequence domains: Tetratricopeptide repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43
Unit cell:
a: 105.13Å b: 105.13Å c: 98.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

The diverse and expanding role of mass spectrometry in structural and molecular biology.
Lössl et al. (2016)
10 more

3 mentions without citation

Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.
Male et al. (2015)
2 more