› 5aim

X-ray diffraction
1.4Å resolution

Crystal structure of T138 central eWH domain

Released:
DOI: 10.2210/pdb5aim/pdb
PDB entry 5aim coloured by chain, front view.
PDB entry 5aim coloured by chain, side view.
PDB entry 5aim coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.
Male G, von Appen A, Glatt S, Taylor NM, Cristovao M, Groetsch H, Beck M, Müller CW
Nat Commun 6 7387 (2015)
PMID: 26060179

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor tau 138 kDa subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 11.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P34111 (Residues: 546-641; Coverage: 8%)
Gene names: FUN24, TFC3, TSV115, YAL001C
Sequence domains: Transcription factor tau 138 kDa subunit, extended winged helix

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: R32
Unit cell:
a: 129.09Å b: 129.09Å c: 68.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.175 0.198
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The diverse and expanding role of mass spectrometry in structural and molecular biology.
Lössl et al. (2016)
10 more

1 mention without citation

Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.
Male et al. (2015)