› 5aiz

X-ray diffraction
1.7Å resolution

The PIAS-like coactivator Zmiz1 is a direct and selective cofactor of Notch1 in T-cell development and leukemia

Released:
DOI: 10.2210/pdb5aiz/pdb
PDB entry 5aiz coloured by chain, front view.
PDB entry 5aiz coloured by chain, side view.
PDB entry 5aiz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.
Pinnell N, Yan R, Cho HJ, Keeley T, Murai MJ, Liu Y, Alarcon AS, Qin J, Wang Q, Kuick R, Elenitoba-Johnson KS, Maillard I, Samuelson LC, Cierpicki T, Chiang MY
Immunity (2015)
PMID: 26522984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193923 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Zinc finger MIZ domain-containing protein 1 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ULJ6 (Residues: 1-118; Coverage: 11%)
Gene names: KIAA1224, RAI17, ZIMP10, ZMIZ1
Sequence domains: Zmiz1 N-terminal tetratricopeptide repeat domain

Ligands and Environments

3 bound ligands:
Ligand UNX 1 x UNX
Ligand EDO 1 x EDO
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 43.472Å b: 45.04Å c: 57.518Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.158 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Notch Signaling in Development, Tissue Homeostasis, and Disease.
Siebel et al. (2017)
4 more

1 mention without citation

The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.
Pinnell et al. (2015)