› 5aji

X-ray diffraction
2.99Å resolution

MscS D67R1 high resolution

Released:
DOI: 10.2210/pdb5aji/pdb
PDB entry 5aji coloured by chain, front view.
PDB entry 5aji coloured by chain, side view.
PDB entry 5aji coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The role of lipids in mechanosensation.
Pliotas C, Dahl AC, Rasmussen T, Mahendran KR, Smith TK, Marius P, Gault J, Banda T, Rasmussen A, Miller S, Robinson CV, Bayley H, Sansom MS, Booth IR, Naismith JH
Nat Struct Mol Biol 22 991-8 (2015)
PMID: 26551077

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small-conductance mechanosensitive channel Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 286 amino acids
Theoretical weight: 31.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C0S1 (Residues: 1-286; Coverage: 100%)
Gene names: JW2891, b2924, mscS, yggB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand OCT 2 x OCT
Ligand HEX 10 x HEX
Ligand D10 3 x D10
1 modified residue:
Ligand R1A 7 x R1A

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 126.33Å b: 149.09Å c: 173.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.244 0.263
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Touch, Tension, and Transduction - The Function and Regulation of Piezo Ion Channels.
Wu et al. (2017)
19 more

10 mentions without citation

Lipid interaction sites on channels, transporters and receptors: Recent insights from molecular dynamics simulations.
Hedger et al. (2016)
9 more