› 5anh

X-ray diffraction
2.49Å resolution

CRYSTAL STRUCTURE OF LACCASE FROM BASIDIOMYCETE PM1 (CECT 2971)

Released:
DOI: 10.2210/pdb5anh/pdb
PDB entry 5anh coloured by chain, front view.
PDB entry 5anh coloured by chain, side view.
PDB entry 5anh coloured by chain, top view.
Source organism: basidiomycete PM1
Primary publication:
Re-Designing the Substrate Binding Pocket of Laccase for Enhanced Oxidation of Sinapic Acid
Pardo I, Santiago G, Gentili P, Lucas F, Monza E, Medrano FJ, Romero A, Galli C, Martinez AT, Guallar V, Camarero S
Catal Sci Technol 6 3900- (2016)

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 496 amino acids
Theoretical weight: 53.27 KDa
Source organism: basidiomycete PM1
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12571 (Residues: 22-517; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 12 x CU
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 101.44Å b: 175.66Å c: 103.7Å
α: 90° β: 104.16° γ: 90°
R-values:
R R work R free
0.197 0.192 0.273
Expression system: Saccharomyces cerevisiae

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