› 5b2m

X-ray diffraction
3.06Å resolution

A crucial role of Cys218 in the stabilization of an unprecedented auto-inhibition form of MAP2K7

Released:
DOI: 10.2210/pdb5b2m/pdb
PDB entry 5b2m coloured by chain, front view.
PDB entry 5b2m coloured by chain, side view.
PDB entry 5b2m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A crucial role of Cys218 in configuring an unprecedented auto-inhibition form of MAP2K7.
Sogabe Y, Hashimoto T, Matsumoto T, Kirii Y, Sawa M, Kinoshita T
Biochem Biophys Res Commun 473 476-81 (2016)
PMID: 26987717

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 7 Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14733 (Residues: 102-419; Coverage: 76%)
Gene names: JNKK2, MAP2K7, MEK7, MKK7, PRKMK7, SKK4
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P6122
Unit cell:
a: 71.603Å b: 71.603Å c: 264.44Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.291 0.288 0.364
Expression system: Escherichia coli

Quick links

Citations

1 review citation

MKK7, the essential regulator of JNK signaling involved in cancer cell survival: a newly emerging anticancer therapeutic target.
Park et al. (2019)
4 other articles