› 5c8d

X-ray diffraction
2.8Å resolution

Crystal structure of full-length Thermus thermophilus CarH bound to adenosylcobalamin (dark state)

Released:
DOI: 10.2210/pdb5c8d/pdb
PDB entry 5c8d coloured by chain, front view.
PDB entry 5c8d coloured by chain, side view.
PDB entry 5c8d coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural basis for gene regulation by a B12-dependent photoreceptor.
Jost M, Fernández-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elías-Arnanz M, Drennan CL
Nature 526 536-41 (2015)
PMID: 26416754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181008 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable transcriptional regulator Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 305 amino acids
Theoretical weight: 33.32 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 1-285; Coverage: 100%)
Gene name: TT_P0056
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand B12 8 x B12
Ligand 5AD 8 x 5AD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 78.74Å b: 79.72Å c: 118.45Å
α: 90.73° β: 96.61° γ: 117.29°
R-values:
R R work R free
0.186 0.183 0.23
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
18 more

6 mentions without citation

A New Facet of Vitamin B12: Gene Regulation by Cobalamin-Based Photoreceptors.
Padmanabhan et al. (2017)
5 more