› 5c8f

X-ray diffraction
2.65Å resolution

Crystal structure of light-exposed full-length Thermus thermophilus CarH bound to cobalamin

Released:
DOI: 10.2210/pdb5c8f/pdb
PDB entry 5c8f coloured by chain, front view.
PDB entry 5c8f coloured by chain, side view.
PDB entry 5c8f coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural basis for gene regulation by a B12-dependent photoreceptor.
Jost M, Fernández-Zapata J, Polanco MC, Ortiz-Guerrero JM, Chen PY, Kang G, Padmanabhan S, Elías-Arnanz M, Drennan CL
Nature 526 536-41 (2015)
PMID: 26416754

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-181007 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable transcriptional regulator Chain: A
Molecule details ›
Chain: A
Length: 305 amino acids
Theoretical weight: 33.32 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746J7 (Residues: 1-285; Coverage: 100%)
Gene name: TT_P0056
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand B12 1 x B12
Ligand CL 2 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4122
Unit cell:
a: 126.91Å b: 126.91Å c: 149.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.203
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
17 more

8 mentions without citation

A New Facet of Vitamin B12: Gene Regulation by Cobalamin-Based Photoreceptors.
Padmanabhan et al. (2017)
7 more