› 5ca7

X-ray diffraction
2.52Å resolution

Human DNA polymerase lambda- MgdGTP binary and complex with 6 paired DNA

Released:
DOI: 10.2210/pdb5ca7/pdb
PDB entry 5ca7 coloured by chain, front view.
PDB entry 5ca7 coloured by chain, side view.
PDB entry 5ca7 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ.
Liu MS, Tsai HY, Liu XX, Ho MC, Wu WJ, Tsai MD
J Am Chem Soc 138 2389-98 (2016)
PMID: 26836966

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193722 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase lambda Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 242-575; Coverage: 58%)
Gene name: POLL
Sequence domains:
Structure domains:
DNA (5'-D(*CP*AP*GP*TP*AP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*TP*AP*CP*TP*G)-3') Chain: T
Molecule details ›
Chain: T
Length: 6 nucleotides
Theoretical weight: 1.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand DGT 1 x DGT
Ligand MG 1 x MG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P6522
Unit cell:
a: 84.113Å b: 84.113Å c: 399.442Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.246
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Nonhomologous DNA end-joining for repair of DNA double-strand breaks.
Pannunzio et al. (2018)
1 more