› 5cbp

X-ray diffraction
2.36Å resolution

Crystal Structure of Conjoint Pyrococcus furiosus L-asparaginase at 37 degree C

Released:
DOI: 10.2210/pdb5cbp/pdb
PDB entry 5cbp coloured by chain, front view.
PDB entry 5cbp coloured by chain, side view.
PDB entry 5cbp coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Heat induces end to end repetitive association in P. furiosus L-asparaginase which enables its thermophilic property.
Sharma P, Tomar R, Yadav SS, Badmalia MD, Nath SK, Kundu B
Sci Rep 10 21702 (2020)
PMID: 33303914

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186321 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
L-asparaginase Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 19.89 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 1-182; Coverage: 56%)
Gene name: PF2047
Sequence domains: Asparaginase, N-terminal
Structure domains: L-asparaginase, N-terminal domain
L-asparaginase Chain: B
Molecule details ›
Chain: B
Length: 127 amino acids
Theoretical weight: 13.9 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TZE8 (Residues: 202-326; Coverage: 38%)
Gene name: PF2047
Sequence domains: Glutaminase/Asparaginase C-terminal domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand FLC 1 x FLC
Ligand GOL 6 x GOL
Ligand IPA 3 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P6522
Unit cell:
a: 90.555Å b: 90.555Å c: 190.041Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Thermo-L-Asparaginases: From the Role in the Viability of Thermophiles and Hyperthermophiles at High Temperatures to a Molecular Understanding of Their Thermoactivity and Thermostability.
Dumina et al. (2023)

1 mention without citation

Heat induces end to end repetitive association in P. furiosus L-asparaginase which enables its thermophilic property.
Sharma et al. (2020)