› 5cc1

X-ray diffraction
2.3Å resolution

S425G Glucocorticoid receptor DNA binding domain - (+)GRE complex

Released:
DOI: 10.2210/pdb5cc1/pdb
PDB entry 5cc1 coloured by chain, front view.
PDB entry 5cc1 coloured by chain, side view.
PDB entry 5cc1 coloured by chain, top view.
Source organisms:
Primary publication:
Distal substitutions drive divergent DNA specificity among paralogous transcription factors through subdivision of conformational space.
Hudson WH, Kossmann BR, de Vera IM, Chuo SW, Weikum ER, Eick GN, Thornton JW, Ivanov IN, Kojetin DJ, Ortlund EA
Proc Natl Acad Sci U S A 113 326-31 (2016)
PMID: 26715749

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118469 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Glucocorticoid receptor Chains: A, B, W, X
Molecule details ›
Chains: A, B, W, X
Length: 114 amino acids
Theoretical weight: 12.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04150 (Residues: 417-506; Coverage: 12%)
Gene names: GRL, NR3C1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*GP*AP*GP*TP*GP*TP*TP*CP*TP*G)-3') Chains: C, Z
Molecule details ›
Chains: C, Z
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*GP*AP*AP*CP*AP*CP*TP*CP*TP*GP*TP*TP*CP*TP*G)-3') Chains: D, Y
Molecule details ›
Chains: D, Y
Length: 18 nucleotides
Theoretical weight: 5.47 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 176.721Å b: 39.122Å c: 132.099Å
α: 90° β: 101.1° γ: 90°
R-values:
R R work R free
0.246 0.245 0.279
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Glucocorticoid receptor control of transcription: precision and plasticity via allostery.
Weikum et al. (2017)
4 more

2 mentions without citation

The Biologist's Guide to the Glucocorticoid Receptor's Structure.
Deploey et al. (2023)
1 more