› 5cef

X-ray diffraction
2.6Å resolution

Cystal structure of aspartate semialdehyde dehydrogenase from Cryptococcus neoformans

Released:
DOI: 10.2210/pdb5cef/pdb
PDB entry 5cef coloured by chain, front view.
PDB entry 5cef coloured by chain, side view.
PDB entry 5cef coloured by chain, top view.
Source organism: Cryptococcus neoformans var. neoformans JEC21
Primary publication:
Structure of a fungal form of aspartate semialdehyde dehydrogenase from Cryptococcus neoformans.
Dahal G, Viola RE
Acta Crystallogr F Struct Biol Commun 71 1365-71 (2015)
PMID: 26527262

Function and Biology Details

Reaction catalysed: 
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177345 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
aspartate-semialdehyde dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 375 amino acids
Theoretical weight: 40.32 KDa
Source organism: Cryptococcus neoformans var. neoformans JEC21
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KPK7 (Residues: 1-365; Coverage: 100%)
Gene name: CNA02450
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 76.59Å b: 130.96Å c: 91.94Å
α: 90° β: 92.87° γ: 90°
R-values:
R R work R free
0.183 0.181 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

2 citation in other articles

A Fragment Library Screening Approach to Identify Selective Inhibitors against an Essential Fungal Enzyme.
Dahal et al. (2018)
1 more

2 mentions without citation

Structure of a fungal form of aspartate semialdehyde dehydrogenase from Cryptococcus neoformans.
Dahal et al. (2015)
1 more