› 5cuf

X-ray diffraction
3.5Å resolution

X-ray crystal structure of SeMet human Sestrin2

Released:
DOI: 10.2210/pdb5cuf/pdb
PDB entry 5cuf coloured by chain, front view.
PDB entry 5cuf coloured by chain, side view.
PDB entry 5cuf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Janus-faced Sestrin2 controls ROS and mTOR signalling through two separate functional domains.
Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, Jakob U, Lee JH, Cho US
Nat Commun 6 10025 (2015)
PMID: 26612684

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sestrin-2 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 483 amino acids
Theoretical weight: 55.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P58004 (Residues: 1-480; Coverage: 100%)
Gene names: Hi95, SESN2, SEST2
Sequence domains: PA26 p53-induced protein (sestrin)

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 65 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I23
Unit cell:
a: 292.68Å b: 292.68Å c: 292.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.235 0.272
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Nutrient sensing and TOR signaling in yeast and mammals.
González et al. (2017)
24 more

3 mentions without citation

Biochemical Basis of Sestrin Physiological Activities.
Ho et al. (2016)
2 more