› 5d2d

X-ray diffraction
2.1Å resolution

Crystal structure of human 14-3-3 zeta in complex with CFTR R-domain peptide pS753-pS768

Released:
DOI: 10.2210/pdb5d2d/pdb
PDB entry 5d2d coloured by chain, front view.
PDB entry 5d2d coloured by chain, side view.
PDB entry 5d2d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Characterization and small-molecule stabilization of the multisite tandem binding between 14-3-3 and the R domain of CFTR.
Stevers LM, Lam CV, Leysen SF, Meijer FA, van Scheppingen DS, de Vries RM, Carlile GW, Milroy LG, Thomas DY, Brunsveld L, Ottmann C
Proc Natl Acad Sci U S A 113 E1152-61 (2016)
PMID: 26888287

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + closed Cl(-) channel = ADP + phosphate + open Cl(-) channel
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146642 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 230 amino acids
Theoretical weight: 26.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Cystic fibrosis transmembrane conductance regulator Chain: C
Molecule details ›
Chain: C
Length: 28 amino acids
Theoretical weight: 3.26 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P13569 (Residues: 747-774; Coverage: 2%)
Gene names: ABCC7, CFTR

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand CL 4 x CL
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 112.862Å b: 112.862Å c: 158.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.22
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Modulators of 14-3-3 Protein-Protein Interactions.
Stevers et al. (2018)
15 more

4 mentions without citation

Recent Strategic Advances in CFTR Drug Discovery: An Overview.
Rusnati et al. (2020)
3 more