› 5d4p

X-ray diffraction
2.2Å resolution

Structure of CPII bound to ADP and bicarbonate, from Thiomonas intermedia K12

Released:
DOI: 10.2210/pdb5d4p/pdb
PDB entry 5d4p coloured by chain, front view.
PDB entry 5d4p coloured by chain, side view.
PDB entry 5d4p coloured by chain, top view.
Source organism: Thiomonas arsenitoxydans
Primary publication:
A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
Wheatley NM, Eden KD, Ngo J, Rosinski JS, Sawaya MR, Cascio D, Collazo M, Hoveida H, Hubbell WL, Yeates TO
J Mol Biol 428 4013-4030 (2016)
PMID: 27464895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrogen regulatory protein P-II GlnB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 108 amino acids
Theoretical weight: 11.95 KDa
Source organism: Thiomonas arsenitoxydans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6CQJ8 (Residues: 1-108; Coverage: 100%)
Gene names: J0I24_06500, THI_0133
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand BCT 7 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 49.81Å b: 76.16Å c: 87.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.191 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

An overview on the recently discovered iota-carbonic anhydrases.
Nocentini et al. (2021)
4 other articles

1 mention without citation

A PII-Like Protein Regulated by Bicarbonate: Structural and Biochemical Studies of the Carboxysome-Associated CPII Protein.
Wheatley et al. (2016)