› 5diz

X-ray diffraction
3.2Å resolution

Crystal Structure of nuclear proteinaceous RNase P 2 (PRORP2) from A. thaliana

Released:
DOI: 10.2210/pdb5diz/pdb
PDB entry 5diz coloured by chain, front view.
PDB entry 5diz coloured by chain, side view.
PDB entry 5diz coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Nuclear Protein-Only Ribonuclease P2 Structure and Biochemical Characterization Provide Insight into the Conserved Properties of tRNA 5' End Processing Enzymes.
Karasik A, Shanmuganathan A, Howard MJ, Fierke CA, Koutmos M
J Mol Biol 428 26-40 (2016)
PMID: 26655022

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinaceous RNase P 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 531 amino acids
Theoretical weight: 59.58 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q680B9 (Residues: 1-528; Coverage: 100%)
Gene names: At2g16650, PRORP2, T24I21.6
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P1
Unit cell:
a: 69.98Å b: 76.993Å c: 80.112Å
α: 72.66° β: 64.11° γ: 77.76°
R-values:
R R work R free
0.23 0.228 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

How RNases Shape Mitochondrial Transcriptomes.
Cartalas et al. (2022)
1 more

5 mentions without citation

The Diversity of Ribonuclease P: Protein and RNA Catalysts with Analogous Biological Functions.
Klemm et al. (2016)
4 more