› 5dnw

X-ray diffraction
2.02Å resolution

Crystal structure of KAI2-like protein from Striga (apo state 1)

Released:
DOI: 10.2210/pdb5dnw/pdb
PDB entry 5dnw coloured by chain, front view.
PDB entry 5dnw coloured by chain, side view.
PDB entry 5dnw coloured by chain, top view.
Source organism: Striga hermonthica
Primary publication:
Structural basis of unique ligand specificity of KAI2-like protein from parasitic weed Striga hermonthica.
Xu Y, Miyakawa T, Nakamura H, Nakamura A, Imamura Y, Asami T, Tanokura M
Sci Rep 6 31386 (2016)
PMID: 27507097

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.24 KDa
Source organism: Striga hermonthica
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3PN85 (Residues: 1-269; Coverage: 98%)
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand FMT 7 x FMT
Ligand EDO 2 x EDO
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P6122
Unit cell:
a: 75.94Å b: 75.94Å c: 181.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.177 0.225
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Strigolactone Signaling and Evolution.
Waters et al. (2017)
11 more

2 mentions without citation

Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga.
Xu et al. (2018)
1 more