5doi

X-ray diffraction
2.2Å resolution

Crystal structure of Tetrahymena p45N and p19

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-112352 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Telomerase-associated protein of 19 kDa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 19.37 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2CVN7 (Residues: 2-164; Coverage: 99%)
Gene names: TAP19, TTHERM_00658760
Telomerase-associated protein of 45 kDa Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 128 amino acids
Theoretical weight: 14.64 KDa
Source organism: Tetrahymena thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6JXI5 (Residues: 2-129; Coverage: 34%)
Gene names: TAP45, TTHERM_00083360

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 44.192Å b: 125.828Å c: 180.167Å
α: 90° β: 90.04° γ: 90°
R-values:
R R work R free
0.232 0.226 0.264
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli