5dqz

X-ray diffraction
2.7Å resolution

Crystal Structure of Cas-DNA-PAM complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-118586 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 305 amino acids
Theoretical weight: 33.24 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46896 (Residues: 1-305; Coverage: 100%)
Gene names: JW2725, b2755, cas1, ygbT
Sequence domains: CRISPR associated protein Cas1
Structure domains:
CRISPR-associated endoribonuclease Cas2 Chains: E, F
Molecule details ›
Chains: E, F
Length: 94 amino acids
Theoretical weight: 10.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P45956 (Residues: 1-94; Coverage: 100%)
Gene names: JW5438, b2754, cas2, ygbF
Sequence domains: CRISPR-associated protein (Cas_Cas2CT1978)
DNA (36-MER) Chain: G
Molecule details ›
Chain: G
Length: 36 nucleotides
Theoretical weight: 11.02 KDa
DNA (36-MER) Chain: H
Molecule details ›
Chain: H
Length: 36 nucleotides
Theoretical weight: 10.98 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 73.771Å b: 193.38Å c: 193.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.224
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli