› 5dui

X-ray diffraction
2.31Å resolution

Identification of a new FoxO1 binding site that precludes CREB binding at the glucose-6-phosphatase catalytic subunit gene promoter

Released:
DOI: 10.2210/pdb5dui/pdb
PDB entry 5dui coloured by chain, front view.
PDB entry 5dui coloured by chain, side view.
PDB entry 5dui coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures reveal a new and novel FoxO1 binding site within the human glucose-6-phosphatase catalytic subunit 1 gene promoter.
Singh P, Han EH, Endrizzi JA, O'Brien RM, Chi YI
J Struct Biol 198 54-64 (2017)
PMID: 28223045

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118594 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12778 (Residues: 151-259; Coverage: 17%)
Gene names: FKHR, FOXO1, FOXO1A
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*TP*TP*TP*CP*TP*AP*TP*TP*TP*TP*AP*CP*GP*TP*AP*AP*AP*TP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*TP*GP*AP*TP*TP*TP*AP*CP*GP*TP*AP*AP*AP*AP*TP*AP*GP*AP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.5 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 46.61Å b: 79.026Å c: 48.266Å
α: 90° β: 90.79° γ: 90°
R-values:
R R work R free
0.21 0.208 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The FOXO's Advantages of Being a Family: Considerations on Function and Evolution.
Schmitt-Ney M. (2020)
1 more

3 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
2 more