› 5dvx

X-ray diffraction
1.6Å resolution

Crystal structure of the catalytic-domain of human carbonic anhydrase IX at 1.6 angstrom resolution

Released:
DOI: 10.2210/pdb5dvx/pdb
PDB entry 5dvx coloured by chain, front view.
PDB entry 5dvx coloured by chain, side view.
PDB entry 5dvx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The Structure of Carbonic Anhydrase IX Is Adapted for Low-pH Catalysis.
Mahon BP, Bhatt A, Socorro L, Driscoll JM, Okoh C, Lomelino CL, Mboge MY, Kurian JJ, Tu C, Agbandje-McKenna M, Frost SC, McKenna R
Biochemistry 55 4642-53 (2016)
PMID: 27439028

Function and Biology Details

Reaction catalysed: 
H(2)CO(3) = CO(2) + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbonic anhydrase 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 28.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16790 (Residues: 140-399; Coverage: 62%)
Gene names: CA9, G250, MN
Sequence domains: Eukaryotic-type carbonic anhydrase
Structure domains: Alpha carbonic anhydrase

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand GOL 6 x GOL
Ligand CL 3 x CL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 57.877Å b: 102.744Å c: 108.964Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.191
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The role of carbonic anhydrase IX in cancer development: links to hypoxia, acidosis, and beyond.
Pastorekova et al. (2019)
9 more

9 mentions without citation

Carbonic Anhydrases: Role in pH Control and Cancer.
Mboge et al. (2018)
8 more