› 5dwd

X-ray diffraction
1.66Å resolution

Crystal structure of esterase PE8

Released:
DOI: 10.2210/pdb5dwd/pdb
PDB entry 5dwd coloured by chain, front view.
PDB entry 5dwd coloured by chain, side view.
PDB entry 5dwd coloured by chain, top view.
Source organism: Pelagibacterium halotolerans B2
Entry authors: Li J, Huang J

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase/carboxylesterase/thioesterase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 240 amino acids
Theoretical weight: 25.53 KDa
Source organism: Pelagibacterium halotolerans B2
Expression system: Escherichia coli
UniProt:
  • Canonical: G4RFI7 (Residues: 1-219; Coverage: 100%)
Gene name: KKY_2995
Sequence domains: Phospholipase/Carboxylesterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand 1PG 1 x 1PG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 41.772Å b: 73.398Å c: 66.403Å
α: 90° β: 102.37° γ: 90°
R-values:
R R work R free
0.158 0.156 0.2
Expression system: Escherichia coli

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