› 5dx5

X-ray diffraction
2.37Å resolution

Crystal structure of methionine gamma-lyase from Clostridium sporogenes

Released:
DOI: 10.2210/pdb5dx5/pdb
PDB entry 5dx5 coloured by chain, front view.
PDB entry 5dx5 coloured by chain, side view.
PDB entry 5dx5 coloured by chain, top view.
Source organism: Clostridium sporogenes
Primary publication:
Structure of methionine γ-lyase from Clostridium sporogenes.
Revtovich S, Anufrieva N, Morozova E, Kulikova V, Nikulin A, Demidkina T
Acta Crystallogr F Struct Biol Commun 72 65-71 (2016)
PMID: 26750487

Function and Biology Details

Reaction catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-methionine gamma-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 43.58 KDa
Source organism: Clostridium sporogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: J7TA22 (Residues: 1-400; Coverage: 100%)
Gene names: CLOSPO_00030, megL
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P43212
Unit cell:
a: 91.09Å b: 91.09Å c: 175.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.225
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Predictive markers for efficiency of the amino-acid deprivation therapies in cancer.
Pokrovsky et al. (2022)

4 mentions without citation

Canavanine utilization via homoserine and hydroxyguanidine by a PLP-dependent γ-lyase in Pseudomonadaceae and Rhizobiales.
Hauth et al. (2022)
3 more