› 5e3x

X-ray diffraction
2.2Å resolution

Crystal structure of thermostable Carboxypeptidase (FisCP) from Fervidobacterium Islandicum AW-1

Released:
DOI: 10.2210/pdb5e3x/pdb
PDB entry 5e3x coloured by chain, front view.
PDB entry 5e3x coloured by chain, side view.
PDB entry 5e3x coloured by chain, top view.
Source organism: Fervidobacterium islandicum
Primary publication:
Biochemical and structural characterization of a keratin-degrading M32 carboxypeptidase from Fervidobacterium islandicum AW-1.
Lee YJ, Dhanasingh I, Ahn JS, Jin HS, Choi JM, Lee SH, Lee DW
Biochem Biophys Res Commun 468 927-33 (2015)
PMID: 26603937

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid with broad specificity, except for -Pro.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102997 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metal-dependent carboxypeptidase Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 57.32 KDa
Source organism: Fervidobacterium islandicum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A140UHP3 (Residues: 1-489; Coverage: 100%)
Sequence domains: Carboxypeptidase Taq (M32) metallopeptidase
Structure domains: Neurolysin; domain 3

Ligands and Environments

1 bound ligand:
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P21212
Unit cell:
a: 107.105Å b: 56.757Å c: 89.183Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.177 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Microbial Keratinase: Next Generation Green Catalyst and Prospective Applications.
Nnolim et al. (2020)
1 more

4 mentions without citation

Microbial enzymes catalyzing keratin degradation: Classification, structure, function.
Qiu et al. (2020)
3 more