› 5e43

X-ray diffraction
1.71Å resolution

Crystal Structure of Beta-lactamase Sros_5706 from Streptosporangium roseum

Released:
DOI: 10.2210/pdb5e43/pdb
PDB entry 5e43 coloured by chain, front view.
PDB entry 5e43 coloured by chain, side view.
PDB entry 5e43 coloured by chain, top view.
Source organism: Streptosporangium roseum DSM 43021
Entry authors: Kim Y, Hatzos-Skintges C, Endres M, Babnigg G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112342 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 31.7 KDa
Source organism: Streptosporangium roseum DSM 43021
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D2ARB3 (Residues: 31-327; Coverage: 100%)
Gene name: Sros_5706
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand NO3 2 x NO3
Ligand ACT 17 x ACT
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 74.845Å b: 92.932Å c: 85.629Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.159 0.187
Expression system: Escherichia coli BL21(DE3)

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