› 5e4e

X-ray diffraction
3Å resolution

Engineered Interleukin-13 bound to receptor

Released:
DOI: 10.2210/pdb5e4e/pdb
PDB entry 5e4e coloured by chain, front view.
PDB entry 5e4e coloured by chain, side view.
PDB entry 5e4e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Instructive roles for cytokine-receptor binding parameters in determining signaling and functional potency.
Moraga I, Richter D, Wilmes S, Winkelmann H, Jude K, Thomas C, Suhoski MM, Engleman EG, Piehler J, Garcia KC
Sci Signal 8 ra114 (2015)
PMID: 26554818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150222 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-13 Chain: A
Molecule details ›
Chain: A
Length: 113 amino acids
Theoretical weight: 12.46 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P35225 (Residues: 34-146; Coverage: 93%)
Gene names: IL13, NC30
Sequence domains: Interleukin-13
Structure domains: Growth Hormone; Chain: A;
Soluble interleukin-4 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 203 amino acids
Theoretical weight: 23.21 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P24394 (Residues: 26-228; Coverage: 25%)
Gene names: 582J2.1, IL4R, IL4RA
Sequence domains: Interleukin-4 receptor alpha chain, N-terminal
Structure domains: Immunoglobulins
Interleukin-13 receptor subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 318 amino acids
Theoretical weight: 36.57 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P78552 (Residues: 23-340; Coverage: 78%)
Gene names: IL13R, IL13RA, IL13RA1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Unit cell:
a: 62.59Å b: 69.37Å c: 186.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.288
Expression system: Trichoplusia ni

Quick links

Citations

7 review citations

Mechanisms of immunomodulation by mammalian and viral decoy receptors: insights from structures.
Felix et al. (2017)
6 more

1 mention without citation

Interleukin-13 peptide vaccine induces protective humoral immunity in murine asthma models.
Wu et al. (2018)