› 5e5s

X-ray diffraction
2.29Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118613 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.09 KDa
Source organism: Sordaria macrospora k-hell
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: F7WD42 (Residues: 114-415; Coverage: 70%)
Gene name: SMAC_12671
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
Bottom strand DNA Chain: B
Molecule details ›
Chain: B
Length: 25 nucleotides
Theoretical weight: 7.69 KDa
Source organism: synthetic construct
Expression system: Not provided
Top strand DNA left site Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*CP*AP*GP*GP*TP*GP*TP*AP*CP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand PG0 1 x PG0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 59.589Å b: 68.199Å c: 97.652Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 citation in other articles

Indirect DNA Sequence Recognition and Its Impact on Nuclease Cleavage Activity.
Lambert et al. (2016)
2 more

1 mention without citation

The Structural Basis of Asymmetry in DNA Binding and Cleavage as Exhibited by the I-SmaMI LAGLIDADG Meganuclease.
Shen et al. (2016)