› 5e6f

X-ray diffraction
2.6Å resolution

Canarypox virus resolvase

Released:
DOI: 10.2210/pdb5e6f/pdb
PDB entry 5e6f coloured by chain, front view.
PDB entry 5e6f coloured by chain, side view.
PDB entry 5e6f coloured by chain, top view.
Source organism: Canarypox virus
Primary publication:
Structure and Metal Binding Properties of a Poxvirus Resolvase.
Li H, Hwang Y, Perry K, Bushman F, Van Duyne GD
J Biol Chem 291 11094-104 (2016)
PMID: 27013661

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CNPV261 Holliday junction resolvase protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.58 KDa
Source organism: Canarypox virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6VZ86 (Residues: 1-152; Coverage: 100%)
Gene name: CNPV261
Sequence domains: Poxvirus A22 protein

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand TAR 1 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P43212
Unit cell:
a: 89.117Å b: 89.117Å c: 117.945Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Viral genome packaging terminase cleaves DNA using the canonical RuvC-like two-metal catalysis mechanism.
Xu et al. (2017)
2 more

2 mentions without citation

Structure and Metal Binding Properties of a Poxvirus Resolvase.
Li et al. (2016)
1 more