› 5e8i

X-ray diffraction
3.45Å resolution

Crystal structure of the DNA binding domain of human transcription factor FLI1 in complex with a 10-mer DNA ACCGGAAGTG

Released:
DOI: 10.2210/pdb5e8i/pdb
PDB entry 5e8i coloured by chain, front view.
PDB entry 5e8i coloured by chain, side view.
PDB entry 5e8i coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for Dimerization and DNA Binding of Transcription Factor FLI1.
Hou C, Tsodikov OV
Biochemistry 54 7365-74 (2015)
PMID: 26618620

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118621 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Friend leukemia integration 1 transcription factor Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 128 amino acids
Theoretical weight: 14.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01543 (Residues: 276-399; Coverage: 27%)
Gene name: FLI1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*CP*CP*GP*GP*AP*AP*GP*TP*G)-3') Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Endothia gyrosa
Expression system: Not provided
DNA (5'-D(*CP*AP*CP*TP*TP*CP*CP*GP*GP*T)-3') Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 10 nucleotides
Theoretical weight: 3 KDa
Source organism: Endothia gyrosa
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3121
Unit cell:
a: 86.647Å b: 86.647Å c: 230.282Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.222 0.271
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Hematopoietic transcription factor mutations: important players in inherited platelet defects.
Songdej et al. (2017)
1 more

5 mentions without citation

Structures of mithramycin analogues bound to DNA and implications for targeting transcription factor FLI1.
Hou et al. (2016)
4 more