› 5egn

X-ray diffraction
2.64Å resolution

Est816 as an N-Acyl homoserine lactone degrading enzyme

Released:
DOI: 10.2210/pdb5egn/pdb
PDB entry 5egn coloured by chain, front view.
PDB entry 5egn coloured by chain, side view.
PDB entry 5egn coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Xie W, Liu X, Cao L, Liu Y

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125218 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 271 amino acids
Theoretical weight: 29.54 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I6YRG4 (Residues: 1-261; Coverage: 96%)
Gene name: est816
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 109.806Å b: 78.545Å c: 116.472Å
α: 90° β: 99.37° γ: 90°
R-values:
R R work R free
0.222 0.219 0.264
Expression system: Escherichia coli BL21(DE3)

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