› 5ehk

X-ray diffraction
2.71Å resolution

Crystal structure of tRNA dependent lantibiotic dehydratase MibB from Microbispora sp. 107891

Released:
DOI: 10.2210/pdb5ehk/pdb
PDB entry 5ehk coloured by chain, front view.
PDB entry 5ehk coloured by chain, side view.
PDB entry 5ehk coloured by chain, top view.
Source organism: Microbispora corallina
Primary publication:
Structure and tRNA Specificity of MibB, a Lantibiotic Dehydratase from Actinobacteria Involved in NAI-107 Biosynthesis.
Ortega MA, Hao Y, Walker MC, Donadio S, Sosio M, Nair SK, van der Donk WA
Cell Chem Biol 23 370-380 (2016)
PMID: 26877024

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lantibiotic dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1115 amino acids
Theoretical weight: 121.87 KDa
Source organism: Microbispora corallina
Expression system: Escherichia coli
UniProt:
  • Canonical: E2IHB7 (Residues: 1-1115; Coverage: 100%)
Gene name: mibB
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 85.137Å b: 151.112Å c: 205.744Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.264
Expression system: Escherichia coli

Quick links

Citations

7 review citations

New developments in RiPP discovery, enzymology and engineering.
Montalbán-López et al. (2021)
6 more

6 mentions without citation

Mechanistic Understanding of Lanthipeptide Biosynthetic Enzymes.
Repka et al. (2017)
5 more